LOCUS       40924_23794       12709 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB729 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12709)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12709)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12709)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12709)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1303
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1340..1370
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1424..2080
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2425..2727
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2771..2895)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2906..2983
                     /codon_start=1
                     /label=CBP
                     /note="calmodulin-binding peptide"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             3011..3031
                     /codon_start=1
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
                     /translation="ENLYFQG"
     CDS             3071..3244
                     /codon_start=1
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             3248..3418
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
                     EAKKLNGAQAPK"
     terminator      3473..3725
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3759..3775)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3783..3799
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3807..3837)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3852..3873)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3909..4161)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4199..5494)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61244.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(4199..5494)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5546..5725)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6231..6255)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6776..7564
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7813..8401
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8587..8727)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9071..9265)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9334..10404)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10836..11462)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1201 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1261 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1321 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1381 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1441 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1501 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1561 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1621 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1681 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1741 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1801 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1861 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1921 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     1981 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2041 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2101 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2161 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     2221 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2281 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2341 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2401 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2461 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2521 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2581 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2641 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2701 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2761 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2821 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2881 cttgtacaaa gtggttgata acagcaagag aagatggaaa aagaatttca tagccgtctc
     2941 agcagccaac cgctttaaga aaatctcatc ctccggggca cttgattatg atattccaac
     3001 tactgctagc gagaatttgt attttcaggg tgagctcaaa accgcggctc ttgcgcaaca
     3061 cgatgaagcc gtggacaaca aattcaacaa agaacaacaa aacgcgttct atgagatctt
     3121 acatttacct aacttaaacg aagaacaacg aaacgccttc atccaaagtt taaaagatga
     3181 cccaagccaa agcgctaacc ttttagcaga agctaaaaag ctaaatgatg ctcaggcgcc
     3241 gaaagtagac aacaaattca acaaagaaca acaaaacgcg ttctatgaga tcttacattt
     3301 acctaactta aacgaagaac aacgaaacgc cttcatccaa agtttaaaag atgacccaag
     3361 ccaaagcgct aaccttttag cagaagctaa aaagctaaat ggtgctcagg cgccgaaagt
     3421 agacgcgaat tccgcgggga agtcaacctg agcttagagc tcgaatttcc ccgatcgttc
     3481 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     3541 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     3601 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     3661 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     3721 agatcgggaa ttggttccgg aaccaattcg taatcatggt catagctgtt tcctgtgtga
     3781 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     3841 tggggtgcct aatgagtgag ctaactcaca ttaggctgaa ttaggcgcgc ctatttctat
     3901 taattcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg
     3961 ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac
     4021 ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa
     4081 cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta
     4141 ttgccaaatg tttgaacgat cggggaaatt cgcctcgagt gacttctctg attgcagact
     4201 atttatacca accagcaaga aagtgtcgaa gtatgaagca gaaggcgcag gctagagcct
     4261 ggaaaacgag aagatggatg caaagagact tttctgattt aggaccaaac aacctcaagt
     4321 aaacattgac gagtgtccca tcttttgtgc cggtcagtaa tccggtagca ggatcatacc
     4381 ttgggagacg atggcgcgga catttcacaa tcccagcaag ctgaggaagc gataatagaa
     4441 ggttcagaac ttgaggaaga aagaagatga ggagggtttc actgaagtga cccagaatac
     4501 caacaactgc catagtcatt ccggcgaaga ctgtgtaagt gtctccaaca aaaactgaag
     4561 aaggatacca attgtaagca agcattgcca aggatgtggc catcagcggt tgagtaagaa
     4621 atatcgagaa ggcatgagct tggtgatact cgggatcaac agatgctccg atttgcataa
     4681 cgttgtgtat caggatagca gcggcaataa caactgtttg accgatttca aggccattca
     4741 gaccagcgtg gatgttaata gagtttgtac aaaagacagc aagtagcccc atatataact
     4801 tatatatcct tcctagatta agcacttcca agccaatata agcaacaaga ggttttggta
     4861 taacaatagt tgtatgacca gcataagcca tcaaaagtgg aagggtagca aaagatggca
     4921 ggacaagttt tactctccaa ggcacatcaa ggacatcatc tacaaatcca agcaaaatca
     4981 tgaagcaaat agatgctagt gctgcattat actccacaag ccaattagaa tcttcagtga
     5041 agttaaaata ctgaaatatt atcgccacga tcaaaaagac aataccaaca acaataccca
     5101 atgactctgg cactttaata tctccttgag gcgtgcctct tttgttgata tcaaaaccaa
     5161 acatgttacg tctcagaacg tatctggcag taacaggaat cattttgaga gtgacgaaaa
     5221 acccaaccaa gctaagcccc gcgttaatta gaatcgatcg cttgagttca ttatcaacct
     5281 tgtagtgaaa acagagaagg tatagataga gcgaacagag aagagtagag atcacgaaga
     5341 tcactcctaa tttcggcggc gcgagacgaa attcctcacc ggaagcagaa accgtctttc
     5401 gggtcatctt ctgctccgat ggcgctgagt tgggttccga cgggtccggt ttgttgggaa
     5461 ttgagatcga tgaagctctc ttacgagctg ccatatctta ttgccccccg ggatcagatc
     5521 tggattgaga gtgaatatga gactctaatt ggataccgag gggaatttat ggaacgtcag
     5581 tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg acttttgaac
     5641 gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa cccgcggctg
     5701 agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt
     5761 catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatctt gatcccctgc
     5821 gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct
     5881 taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt
     5941 ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt
     6001 cccttgtcca gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac
     6061 tggctttcta cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc
     6121 gtgaagctga tccgtcgaga ttaatagaaa taaattcagc ctaattcggc gttaattcag
     6181 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
     6241 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
     6301 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
     6361 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
     6421 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
     6481 ctgcctgtga tcaattcggg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     6541 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     6601 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     6661 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     6721 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg
     6781 ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg
     6841 ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct
     6901 gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac
     6961 gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat
     7021 tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc
     7081 agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt
     7141 cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag
     7201 cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct
     7261 atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga
     7321 tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat
     7381 cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc
     7441 cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg
     7501 cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg
     7561 caaataatgt ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact
     7621 caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc
     7681 ttttattatt tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgcat
     7741 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     7801 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     7861 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     7921 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     7981 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8041 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8101 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8161 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8221 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8281 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8341 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8401 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8461 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8521 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8581 agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     8641 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     8701 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     8761 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     8821 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg
     8881 tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg
     8941 ccgtaggcca gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg
     9001 gcgtagggag cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg
     9061 gccagacagt tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt
     9121 ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt
     9181 cccaatgtac ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg
     9241 gttcccaatg tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag
     9301 ggcaatttgc cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat
     9361 caggttgcgg tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc
     9421 gtactccggc aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa
     9481 ctctccggcg ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt
     9541 gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa
     9601 gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat
     9661 ccaatcagct agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt
     9721 gtagcggcta atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt
     9781 cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc
     9841 gtctttctgc tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg
     9901 acggaacacg cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt
     9961 tagatgggaa accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg
    10021 ccctgcggaa acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg
    10081 tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt
    10141 gcccacgtca tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt
    10201 cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc
    10261 ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc
    10321 ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg
    10381 gccggatggt ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg
    10441 cagggccggc agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg
    10501 gggcattcca cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc
    10561 gctaaaattc atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta
    10621 ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt
    10681 gtgatcctcc gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct
    10741 ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt
    10801 gcttttgctc attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg
    10861 gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg
    10921 ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc
    10981 agctcgtacc cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc
    11041 cgcgacacga caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc
    11101 agctccacca ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc
    11161 acgaagtcgg ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg
    11221 atcactacga agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg
    11281 atgccgacaa cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc
    11341 tggggatcgg aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga
    11401 tgggttgcga tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc
    11461 atgcgttccc cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat
    11521 gacgcaagct gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt
    11581 cagcggccaa gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt
    11641 catgcagccg cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca
    11701 tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt
    11761 tcatgcttgt tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa
    11821 tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg
    11881 ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac
    11941 ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt
    12001 agggcggggg ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg
    12061 gtcgatgatt agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg
    12121 gccggccggc gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc
    12181 ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag
    12241 cctggtcact gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg
    12301 gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg
    12361 cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag
    12421 caggccagcg gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt
    12481 ctactttatg cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc
    12541 ctgtcgcgta acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg
    12601 tcagaagccg actgcactat agcagcggag gggttggatc aaagtacttt gatcccgagg
    12661 ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacct
//
